Additional file 1.
Table S1. Reference gene primers tested. BA = Bérubé and Aguilar ; CW 2006 = Callicot and Womack ; RC 2009 = Cawthon . Note that all reference gene primers used in assays II-IV had a CG-clamp (CGGCGGCGGGCGGCGCGGGCTGGGCGG) attached to increase annealing temperature as described in Cawthon (2009). * Caution; this primer pair was used in assay I in which primer efficiency was found to correlate with DNA concentration.
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Olsen et al. BMC Genetics 2012 13:77 doi:10.1186/1471-2156-13-77