Table 3 |
||||
| List of primer pairs | ||||
| Gene | Exon | Primer name | Sequence | R-MPCgb |
| gDNA | ||||
| CCL8 | ex1 | FwPrCL8e1 | 5’ AGCACACGCAGGGTCTTGCT 3’ | 47421-47440§ |
| RvPrCL8e1a | 5’ ATGGCTCCCACTTGGATGGC 3’ | 47692-47711 | ||
| RvPrCL8e1b | 5’ TCGACCCCGTGGGCTGGTAG 3´ | 48091-48110 | ||
| ex2 | FwPrCL8e2a | 5’ GCATCCAGCACGGTGGCTGT 3’ | 48021-48040 | |
| RvPrCL8e2a | 5’ GCCAGCCCTTGCTCCTTGGG 3’ | 48774-48793 | ||
| ex3 | FwPrCL8e3b | 5’ GGCTCCAGGTGCTTCAGCCA 3’ | 48659-48678 | |
| RvPrCL8e3b | 5’AGTACCCAGGGAAGGCTGGG 3’ | 49034-49054 | ||
| CCL13 | ex1 | F1CL13e1 | 5’ TTGGCTCTCCCGTGGCAGCA 3’ | 72054-72073 |
| R1CL13e1 | 5’ GGCCAGCACTATGGCGCAGT 3’ | 72537-72556 | ||
| F9CL13e1 | 5’ AGGCAGCAAGCATGGGAGCG 3’ | 71722-71741 | ||
| R9CL13e1 | 5’ GGGCCCTTTGGCTTAGAAGGCG 3’ | 72226-72206 | ||
| cDNA | ||||
| CCL8 | CDS | FwCCL8_CDS | 5’ CTCCAAGCTCGGCTCCTTG 3’ | 47548-47566 |
| RvCCL8_CDS | 5’ ACTCTGAGTCTCAGTCCAGGTG 3’ | 48990-49011 | ||
| CCL7 | CDS | FwCCL7_CDS | 5’ AGGCTGAGGCCAGCACAGGA 3’ | 13720-13739 |
| RvCCL7_CDS | 5’ TCCTCCCCCATGCCAGTGCA 3’ | 11541-11560 | ||
§ NC_013687.1 REGION:2376421.23767440.
van der Loo et al. BMC Genetics 2012 13:72 doi:10.1186/1471-2156-13-72
