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Open Access Research article

Genetic map of Triticum turgidum based on a hexaploid wheat population without genetic recombination for D genome

Li Zhang1, Jiang-Tao Luo1, Ming Hao1, Lian-Quan Zhang1, Zhong-Wei Yuan1, Ze-Hong Yan1, Ya-Xi Liu1, Bo Zhang2, Bao-Long Liu2, Chun-Ji Liu3, Huai-Gang Zhang2, You-Liang Zheng1 and Deng-Cai Liu12*

Author Affiliations

1 Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China

2 Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China

3 CSIRO Plant Industry, 306 Carmody Road, St Lucia, QLD, 4067, Australia

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BMC Genetics 2012, 13:69  doi:10.1186/1471-2156-13-69

Published: 13 August 2012

Abstract

Background

A synthetic doubled-haploid hexaploid wheat population, SynDH1, derived from the spontaneous chromosome doubling of triploid F1 hybrid plants obtained from the cross of hybrids Triticum turgidum ssp. durum line Langdon (LDN) and ssp. turgidum line AS313, with Aegilops tauschii ssp. tauschii accession AS60, was previously constructed. SynDH1 is a tetraploidization-hexaploid doubled haploid (DH) population because it contains recombinant A and B chromosomes from two different T. turgidum genotypes, while all the D chromosomes from Ae. tauschii are homogenous across the whole population. This paper reports the construction of a genetic map using this population.

Results

Of the 606 markers used to assemble the genetic map, 588 (97%) were assigned to linkage groups. These included 513 Diversity Arrays Technology (DArT) markers, 72 simple sequence repeat (SSR), one insertion site-based polymorphism (ISBP), and two high-molecular-weight glutenin subunit (HMW-GS) markers. These markers were assigned to the 14 chromosomes, covering 2048.79 cM, with a mean distance of 3.48 cM between adjacent markers. This map showed good coverage of the A and B genome chromosomes, apart from 3A, 5A, 6A, and 4B. Compared with previously reported maps, most shared markers showed highly consistent orders. This map was successfully used to identify five quantitative trait loci (QTL), including two for spikelet number on chromosomes 7A and 5B, two for spike length on 7A and 3B, and one for 1000-grain weight on 4B. However, differences in crossability QTL between the two T. turgidum parents may explain the segregation distortion regions on chromosomes 1A, 3B, and 6B.

Conclusions

A genetic map of T. turgidum including 588 markers was constructed using a synthetic doubled haploid (SynDH) hexaploid wheat population. Five QTLs for three agronomic traits were identified from this population. However, more markers are needed to increase the density and resolution of this map in the future study.

Keywords:
Allopolyploid; Crossability; Doubled haploid; Segregation distortion