Mutation rates of mcm2 alleles and strains with gene deletions that suppress mcm2AA. The mutation rates of MCM2 and mcm2AA strains with and without gene deletions that suppress mcm2AA were calculated as described [39,40] using the CAN1 forward mutation assay. Dark grey bars are the deletions in the MCM2 background; white bars are with mcm2AA and the light grey bar (far left) is the mcm2EE strain. The error bars represent the upper and lower confidence limits (95%) of the mutation rates and were calculated from the 95% confidence limits of the median determined from the binomial distribution .
Stead et al. BMC Genetics 2012 13:36 doi:10.1186/1471-2156-13-36