Sensitivities of mcm2 alleles to genotoxic agents. The growth of phosphorylation site mutations in mcm2 was compared on plates containing genotoxic agents. The mec2-1 strain  was utilized to control for plate quality. A) The growth of mcm2AA and MCM2 strains was compared on YPD and YPD containing 0.03% MMS, 400 μM 5-FU or 200 mM HU by spotting 10-fold serial dilutions of the strains followed by growth at 30°C for the indicated times. B) Similarly, the growth of mcm2AA and MCM2 strains was compared on YPD with and without phleomycin. C) The growth of mcm2EEmcm2S164Emcm2S170E and MCM2 on YPD with 0.03% MMS, 400 μM 5-FU or 200 mM HU.
Stead et al. BMC Genetics 2012 13:36 doi:10.1186/1471-2156-13-36