Open Access Research article

Association between GNRHR, LHR and IGF1 polymorphisms and timing of puberty in male Angus cattle

Juan P Lirón1, Alberto J Prando2, María E Fernández1, María V Ripoli1, Andrés Rogberg-Muñoz1, Daniel E Goszczynski1, Diego M Posik13, Pilar Peral-García1, Andrés Baldo2 and Guillermo Giovambattista1*

Author Affiliations

1 Instituto de Genética Veterinaria (IGEVET), CCT La Plata - CONICET - Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina

2 Departamento de Producción Animal, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina

3 Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Buenos Aires, Argentina

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BMC Genetics 2012, 13:26  doi:10.1186/1471-2156-13-26

Published: 5 April 2012

Additional files

Additional file 1:

Table S1. Gene frequency, Hardy-Weinberg equilibrium (HWE), expected heterozygosities (he), and gene differentiation estimated for GNRHR-SNP5, GNRHR-SNP6, LHR-I499L and IGF1-SnaBI alleles identified by pyrosequencing analysis within each herd and in the whole sample. Table S2. Average estimated puberty age and weigh estimated at 300 days (W 300), with their standard errors (SE) calculated for each herd and year. Results for the two criterions for age of puberty are: i) estimated at Scrotal Circumference (SC) = 28 cm, ii) estimated at Sperm Motility (M) = 10% and Sperm Concentration (C) = 50 million. Table S3. Summary information of genotyped SNPs for gonadotropin-releasing hormone receptor (GNRHR). luteinizing hormone receptor (LHR) and insulin-like growth factor 1 (IGF1) genes. Table S4. Primer sequences and annealing temperatures used for SNP genotyping by pyrosequencing assays.

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