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Open Access Highly Accessed Research article

Development and characterization of Brassica juncea – fruticulosa introgression lines exhibiting resistance to mustard aphid (Lipaphis erysimi Kalt)

Chhaya Atri, Bharti Kumar, Hitesh Kumar, Sarwan Kumar, Sanjula Sharma and Surinder S Banga*

  • * Corresponding author: Surinder S Banga nppbg@pau.edu

  • † Equal contributors

Author Affiliations

Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141 004, India

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BMC Genetics 2012, 13:104  doi:10.1186/1471-2156-13-104

Published: 27 November 2012

Abstract

Background

Mustard aphid is a major pest of Brassica oilseeds. No source for aphid resistance is presently available in Brassica juncea . A wild crucifer, Brassica fruticulosa is known to be resistant to mustard aphid. An artificially synthesized amphiploid, AD-4 (B. fruticulosa × B. rapa var. brown sarson) was developed for use as a bridge species to transfer fruticulosa resistance to B. juncea. Using the selfed backcross we could select a large number of lines with resistance to mustard aphid. This paper reports cytogenetic stability of introgression lines, molecular evidence for alien introgression and their reaction to mustard aphid infestation.

Results

Majority of introgression lines had expected euploid chromosome number(2n= 36), showed normal meiosis and high pollen grain fertility. Well-distributed and transferable simple-sequence repeats (SSR) markers for all the 18 B. juncea chromosomes helped to characterize introgression events. Average proportions of recipient and donor genome in the substitution lines were 49.72 and 35.06%, respectively. Minimum alien parent genome presence (27.29%) was observed in the introgression line, Ad3K-280 . Introgressed genotypes also varied for their resistance responses to mustard aphid infestations under artificial release conditions for two continuous seasons. Some of the test genotypes showed consistent resistant reaction.

Conclusions

B.juncea-fruticulosa introgression set may prove to be a very powerful breeding tool for aphid resistance related QTL/gene discovery and fine mapping of the desired genes/QTLs to facilitate marker assisted transfer of identified gene(s) for mustard aphid resistance in the background of commercial mustard genotypes.

Keywords:
Insect resistance; Indian mustard; Lipaphis erysimi; Wild crucifer; Wide hybridization