Amplification of SVMP cDNAs by PCR. Total cDNA obtained by reverse transcription of venom gland isolated mRNA was submitted to PCR using as primers PRODOM and 3'UTR as described in Methods. Samples were analyzed in 1.5% agarose gels stained with GelRedTM (Biotium). (MM) Molecular markers Biolab ladder; (PCR) amplification product. Note the bands with expected sizes of SVMPs classes P-I, P-II and P-III.
Moura-da-Silva et al. BMC Genetics 2011 12:94 doi:10.1186/1471-2156-12-94