Figure 3.

Loss of NF-κB activation by the Pro153Ser Edaradd mutant protein. 293T cells were transfected with 1.2 μg pNF-κB-luciferase reporter gene plasmid, 2 μg pRL-TK, and the amounts of each expression construct were measured in a reporter assay. The expression levels of luciferase were normalized to those of the internal control. Relative NF-κB activity in wild-type transfected cells increased in a dose-dependent manner, while significantly lower relative NF-κB activity was observed in the Pro153Ser Edaradd-transfected cells. *P < 0.01, **P < 0.001.