Open Access Research article

Polymorphism, selection and tandem duplication of transferrin genes in Atlantic cod (Gadus morhua) - Conserved synteny between fish monolobal and tetrapod bilobal transferrin loci

Øivind Andersen12*, Maria Cristina De Rosa3, Davide Pirolli3, Ave Tooming-Klunderud4, Petra E Petersen5 and Carl André6

Author Affiliations

1 Nofima Marin, P. O. Box 5010, N-1430 Ås, Norway

2 Norwegian University of Life Sciences, P. O. Box 5003, N-1430 Ås, Norway

3 Institute of Chemistry of Molecular Recognition - CNR and Institute of Biochemistry and Clinical Biochemistry, Catholic University of Rome, 00168 Rome, Italy

4 Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biology, University of Oslo, P.O. Box 1066 Blindern, N-0316 Oslo, Norway

5 Aquaculture Research Station of the Faroes, FO-430 Hvalvík, Faroe Islands

6 Department of Marine Ecology-Tjärnö, Gothenburg University, S-45296 Strömstad, Sweden

For all author emails, please log on.

BMC Genetics 2011, 12:51  doi:10.1186/1471-2156-12-51

Published: 25 May 2011

Additional files

Additional file 1:

Figure S1 Alignment of the NE and NW variants of Atlantic cod Tf1 cDNAs. The Atlantic cod Tf1 cDNA derived from two Northeast Atlantic populations was compared with that of a Northwest Atlantic population [44]. The 22 SNPs identified are numbered, and the six analysed SNPs are shown in bold.

Format: DOC Size: 33KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

Figure S2 Map of the Atlantic cod populations examined. Six out of 22 SNPs identified in cod Tf1 were analysed in 14 populations across the North-Atlantic.

Format: DOC Size: 294KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 3:

Table S1 Genotype frequencies for six Atlantic cod Tf1 SNP loci in 14 samples across the North Atlantic. Sample sizes of cod collected at each location in the first row.

Format: DOC Size: 108KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 4:

Table S2 Genetic differentiation among pairs of Atlantic cod samples. Below diagonal are pairwise FST-values, and above diagonal statistical significance values *P < 0.05; **P < 0.01; ***P < 0.001.

Format: DOC Size: 200KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 5:

Table S3 PCR primers for SNP analysis, RT-PCR and WISH. The sequences are shown in 5'-3' direction.

Format: DOC Size: 31KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 6:

Table S4 Accesion numbers of the proteins included in the phylogenetic analysis. Several distinct transferrin genes have been described in some teleost species.

Format: DOC Size: 63KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data