Table 2

Primers for SNP detection in the porcine APOE 5'-regulatory region

Primers

Sequences

Annealing temperature

PCR product length


P1

Upstream: 5'-TCGAGAGGGAGTGAGGGTTAAAA-3'

59°C

538 bp

Downstream: 5'-AGGAGCAGGACCAGAAGG-3'

P2

Upstream: 5'-GGCCTTCTGGTCCTGCTCCT-3'

61°C

455 bp

Downstream: 5'-AACTCCTGCCTCGGTGGTGG-3'

P3

Upstream: 5'-CACCGAGGCAGGAGTTGG-3'

60°C

728 bp

Downstream: 5'-CTTCTGGCTTGCGATTGG-3'


Li et al. BMC Genetics 2011 12:40   doi:10.1186/1471-2156-12-40

Open Data