The T>A transition at position -155 of the porcine APOE gene increases DNA-binding affinity of nuclear protein(s) in porcine fetal fibroblasts. Electrophoretic mobility shift assays were carried out as described in Materials and Methods. Protein-DNA complexes were separated on 5% PAGE and exposed to X-ray film. In competition experiments (lanes 3-6), Nuclear extracts were preincubated in the absence (-) or 10-fold excess (+) or 50-fold excess (++) of unlablled competitor before addition of the 32P-labelled probe. Binding complexes identified with the -155T probe and -155A probe were indicated with arrows.
Li et al. BMC Genetics 2011 12:40 doi:10.1186/1471-2156-12-40