AttC PCR primer design. Primers YB3 and YB4 are shown aligned with a typical attC sequence (the cassette 19-20 junction in this case) from the Vibrio sp. DAT722 array. The dotted line indicates the attC recombination site. YB3 is shown reversed for clarity.
Michael and Labbate BMC Genetics 2010 11:82 doi:10.1186/1471-2156-11-82