Table 8

Estimated reagent and sequencing costs

Reagent

Cost/well

Extended cost Plate 1

Extended cost Plate 2 or 3


Assay

$0.03


Fast Start

$0.34


dNTPs

$0.04


10× Buffer

$0.02


Sub Total

$0.43


X6/5*

$0.52


X8/7#

$0.49


Plastic ware

$0.06

$0.06

$0.06


QIAxcel system

$0.25

$0.25

$0.25


AmPure

$0.07

$0.07

$0.07


Total PCR

reagents

$0.90/patient PCR

$0.87/patient PCR


Big Dye

$0.90

$1.80/patient PCRΘ

$1.80/patient PCR


Buffer

$0.06

$0.12/patient PCR

$0.12/patient PCR


Plate

$0.01

$0.02/patient PCR

$0.02/patient PCR


CleanSeq

$0.30

$0.60/patient well

$0.60/patient PCR


Plate

$0.01

$0.02/patient PCR

$0.02/patient PCR


Total per patient well

$3.46

$3.43


Total per patient

$442.88

$329.28


Add ~20% for waste

$532.00

$395.00


* for one negative control per 5 patients on plate 1; # one negative control per 7 patients on plates 2 or 3; Θ for both Forward and Reverse sequence/PCR.

20% waste value is a conservative estimate of waste established over many years and used by our clinical and research labs in calculating material waste. It includes remaining reagents in large cocktail batches and possible manual repeats.

Bennett et al. BMC Genetics 2009 10:66   doi:10.1186/1471-2156-10-66

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