Simultaneous genotyping of common SNP rs9894946 and rare SNP rs17883532. A. In mutation scanning mode, heterozygous samples for either the first (in red) or the second SNP (in green) have virtually indistinguishable melting curves. B. In genotyping mode using an unlabeled probe for rs9894946, the 3 genotypes are distinguisable (CC in gray, CT in red, TT in blue). C. Mutation scanning of homozygous rs9894946 CC subset reveals heterozygous rs17883532 CT (In green).
Garritano et al. BMC Genetics 2009 10:5 doi:10.1186/1471-2156-10-5