Additional file 2.
Galactose induction of candidate chromosome VI disomes. Galactose induces the appearance of many small Ura+His+ papillae in a strain carrying a modified chromosome VI. Strain KAY628, harbouring the LEU2-marked TUB1 plasmid pKA55, was grown to log phase in raffinose-containing medium, split, and one-half was exposed to galactose for 1.3 culture doublings. 107 cells were spread to plates selecting for Ura+His+Leu+ papillae. Plates were incubated 3 days and photographed. Papillae were picked, colony-purified, then cultured for DNA isolation and array CGH as described in the text.
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Anders et al. BMC Genetics 2009 10:36 doi:10.1186/1471-2156-10-36