Table 1

Singleplex PCR results for the bone (BPE1, HB50) and faecal (HB49) extracts of the Iceman's mtDNA, showing all polymorphisms obtained in separate sets of cloned sequences.

Control Region

Coding Region

P1

P2


HB50

G263G

    16224C

    16311C

    16362C

497T

498delC

1189C

1811G

5193A

8137T

9055A

9698C

9716C

9962A

    10398G

14167T

00100

00207


HB50

00157

00290


HB50

16015

16179


HB50

16074

16231


HB49

C

16131

16300


HB50

C

C

C

16186

16398


BPE1

C

C

C

16192

16402


HB49

C

C

16257

16398


HB50

C

16186

16291


HB50

C

16257

16363


HB50

C

16320

16424


HB50

C

C

00460

00524


HB50

C

01142

01220


HB49

G

01781

01848


HB50

G

01781

01848


HB50

G

05846

05950


HB50

T

08105

08205


HB49

T

08105

08205


BPE1

T

08105

08205


BPE1

A

09029

09095


HB50

C

T

09636

09742


HB50

G

09922

10005


HB50

G

10353

10442


HB49

T

14105

14207


Con.

G

C

C

C

C

C

C

G

G

T

A

C

T

G

G

T


rCRS

A

T

T

T

C

C

T

A

G

C

G

T

T

G

A

C


In this table alleles derived from the revised reference sequence (rCRS) are shown in bold and with gray background. The consensus (Con.) is given above the Cambridge Reference Sequence (rCRS) [23,24] in the last but one row. The alleles 263G, 1189C, 1811G, 9055A, 9698C, 14167T, 16224C and 16311C are typical of haplogroup (hg) K1, lying within macro-haplogroup R of the human mtDNA genealogy [13]. The Iceman's mtDNA is further characterised by 8137T (so far unique within hg K) and 16362C. The last two columns (P1, P2) give the 5 prime position of each primer used for the amplification, full details of which are in additional file 4.

Endicott et al. BMC Genetics 2009 10:29   doi:10.1186/1471-2156-10-29

Open Data