Mutations in genes involved in nonsense mediated decay ameliorate the phenotype of sel-12 mutants with amber stop mutations in Caenorhabditis elegans
1 Nematode Genetics Group, Department of Neuroscience, Institut Pasteur, 25 rue du docteur Roux, Paris, France
2 Current address : Instituto de Neurociencias, CSIC-UMH, Unidad de Neurobiología del Desarrollo, Campus de Sant Joan, Apto 18. 03550 Sant Joan d'Alacant, Alicante, Spain
3 Current address : Unité postulante de Pathogenèse de Helicobacter, Institut Pasteur, 28 rue du docteur Roux, Paris, France
4 Current address : Unité de Génétique Humaine et Fonctions Cognitives, Institut Pasteur, 25 rue du docteur Roux, Paris, France
BMC Genetics 2009, 10:14 doi:10.1186/1471-2156-10-14Published: 20 March 2009
Presenilin proteins are part of a complex of proteins that can cleave many type I transmembrane proteins, including Notch Receptors and the Amyloid Precursor Protein, in the middle of the transmembrane domain. Dominant mutations in the human presenilin genes PS1 and PS2 lead to Familial Alzheimer's disease. Mutations in the Caenorhabditis elegans sel-12 presenilin gene cause a highly penetrant egg-laying defect due to reduction of signalling through the lin-12/Notch receptor. Mutations in six spr genes (for
We show that spr-2 mutations increase the levels of sel-12 transcripts with Premature translation Termination Codons (PTCs) in embryos and L1 larvae. mRNA transcripts from sel-12 alleles with PTCs undergo degradation by a process known as Nonsense Mediated Decay (NMD). However, spr-2 mutations do not appear to affect NMD. Mutations in the smg genes, which are required for NMD, can restore sel-12(PTC) transcript levels and ameliorate the phenotype of sel-12 mutants with amber PTCs. However, the phenotypic suppression of sel-12 by smg genes is nowhere near as strong as the effect of previously characterized spr mutations including spr-2. Consistent with this, we have identified only two mutations in smg genes among the more than 100 spr mutations recovered in genetic screens.
spr-2 mutations do not suppress sel-12 by affecting NMD of sel-12(PTC) transcripts and appear to have a novel mechanism of suppression. The fact that mutations in smg genes can ameliorate the phenotype of sel-12 alleles with amber PTCs suggests that some read-through of sel-12(amber) alleles occurs in smg backgrounds.