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Open Access Highly Accessed Research article

Meiosis genes in Daphnia pulex and the role of parthenogenesis in genome evolution

Andrew M Schurko1, John M Logsdon1 and Brian D Eads2*

Author Affiliations

1 Roy J Carver Center for Comparative Genomics and Department of Biology, The University of Iowa, Iowa City, Iowa 52242, USA

2 The Center for Genomics and Bioinformatics and Department of Biology, Indiana University, Bloomington, Indiana 47405, USA

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BMC Evolutionary Biology 2009, 9:78  doi:10.1186/1471-2148-9-78

Published: 21 April 2009

Additional files

Additional file 1:

Representative images of RT-PCR products (from Additional File 2) subjected to gel electrophoresis. Contains gel images of RT-PCR products amplified with primers detailed in Additional File 3.

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Additional file 2:

Annotated genes in Daphnia pulex associated with meiosis. Protein IDs correspond to gene models assigned by the Joint Genome Institute [113] and their corresponding genomic coordinates. Columns for Meiotic Ovaries (resting egg production by cyclical parthenogens), Obligate Parthenogenetic Ovaries (resting egg production by obligate parthenogens), Males (meiosis; whole animals) and Female Soma (cyclical parthenogens) represent RT-PCR data from these tissues as presence (+) or absence (-) using primers detailed in Additional File 2. EST, Tiling path, and repeat region data for each protein were found on the wFleabase genome browser [94]. N/D is not determined.

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Additional file 3:

Primers used in RT-PCR gene expression studies. Primer sequences, protein ID and genomic coordinates for RT-PCR target genes.

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Additional file 4:

Gene expression patterns and genomic locations corresponding to truncated copies of SMC1 proteins in D. pulex. Contains information about SMC1 psuedogenes and truncated proteins, including RT-PCR and tiling path microarray gene expression data.

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Additional file 5:

Genomic locations of two repetitive regions found near the D. pulex Rec8 paralogs A, B and C. Contains coordinates of repetitive regions near paralogs of meiotic cohesin Rec8.

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