Research article
Meiosis genes in Daphnia pulex and the role of parthenogenesis in genome evolution
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* Corresponding author: Brian D Eads bdeads@indiana.edu
1 Roy J Carver Center for Comparative Genomics and Department of Biology, The University of Iowa, Iowa City, Iowa 52242, USA
2 The Center for Genomics and Bioinformatics and Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
BMC Evolutionary Biology 2009, 9:78 doi:10.1186/1471-2148-9-78
Published: 21 April 2009Additional files
Additional file 1:
Representative images of RT-PCR products (from Additional File 2) subjected to gel electrophoresis. Contains gel images of RT-PCR products amplified with primers detailed in Additional File 3.
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Additional file 2:
Annotated genes in Daphnia pulex associated with meiosis. Protein IDs correspond to gene models assigned by the Joint Genome Institute [113] and their corresponding genomic coordinates. Columns for Meiotic Ovaries (resting egg production by cyclical parthenogens), Obligate Parthenogenetic Ovaries (resting egg production by obligate parthenogens), Males (meiosis; whole animals) and Female Soma (cyclical parthenogens) represent RT-PCR data from these tissues as presence (+) or absence (-) using primers detailed in Additional File 2. EST, Tiling path, and repeat region data for each protein were found on the wFleabase genome browser [94]. N/D is not determined.
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Additional file 3:
Primers used in RT-PCR gene expression studies. Primer sequences, protein ID and genomic coordinates for RT-PCR target genes.
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Additional file 4:
Gene expression patterns and genomic locations corresponding to truncated copies of SMC1 proteins in D. pulex. Contains information about SMC1 psuedogenes and truncated proteins, including RT-PCR and tiling path microarray gene expression data.
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Additional file 5:
Genomic locations of two repetitive regions found near the D. pulex Rec8 paralogs A, B and C. Contains coordinates of repetitive regions near paralogs of meiotic cohesin Rec8.
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