Table 5

Primers used in this study

Locus

Primer

Primer sequence 5' - 3'

Reference

°C

cytb

L-CYT

AAT GAT ATG AAA AAC CAT CGT TGT A

[64]

55

2763

GG(AG) ATT TT(AG) TCG GAG TCT GAT G

this study

2695

CCG ATT CTT CGC ATT CCA CTT

this study

55

2510

GAC CAG (GT)GT ATT (AT)TT TAT ACT AC

C. Roos, pers. comm.

2877

ACG TAA AC(CT) ACG GCT GAA

this study

52

2879

CCT CAG ATT CAT TCT ACT A

this study

cox2

L7553

AAC CAT TTC ATA ACT TTG TCA A

[65]

48

H8320*

CTC TTT AAT CTT TAA CTT AAA AG

[65]

adora3

adora3F

ACC CCC ATG TTT GGC TGG AA

[66]

52

adora3R

GAT AGG GTT CAT CAT GGA GTT

[67]

fiba

Fiba-F

AAG CGC AAA GTC ATA GAA AAA G

[66]

56

Fiba-R

CTA AAG CCC TAC TGC ATG ACC CT

[66]

vWF

vWF-10

GAG CTG GAT GTC CTG GCC ATC CAT GGC AAC

[68]

60

vWF-8

GAG TGC CTT GTC ACT GGT CAT CCC ACT TCA A

[68]

The locus, primer name, primer sequence, reference and used annealing temperature in °C are given. The complete cytb locus was obtained by amplifying two overlapping fragments (primer pairs 1255, 2763 and 2695, 2510). The cytb fragments from museum material were amplified using primer pair 2877, 2879. *slightly modified from Adkins and Honeycutt(1994), fourth base not present in original primer.

Groeneveld et al. BMC Evolutionary Biology 2009 9:30   doi:10.1186/1471-2148-9-30