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Open Access Research article

Molecular evolution of sex-biased genes in the Drosophila ananassae subgroup

Sonja Grath*, John F Baines and John Parsch

Author Affiliations

Department of Biology, University of Munich (LMU), Munich, Germany

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BMC Evolutionary Biology 2009, 9:291  doi:10.1186/1471-2148-9-291

Published: 16 December 2009

Additional files

Additional file 1:

Primers for PCR-amplicon microarrays. Primers were designed to amplify single exons of protein-coding genes using the D. ananassae genome assembly from August 2005 http://genome.ucsc.edu/ webcite. Given is the length of the amplified fragment. The dataset contains 148 candidate genes plus two highly-expressed genes (Adh, RpL23) that were used as controls.

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Additional file 2:

Sex-biased gene expression in D. ananassae compared to D. melanogaster and D. pseudoobscura. The degree of sex-biased gene expression is given as the ratio of male/female expression. For D. ananassae, gene expression ratio was determined from up to two independent experiments. For D. melanogaster, expression data from up to three independent experiments were used.

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Additional file 3:

Comparison D. ananassae/D. melanogaster. For 43 genes, intraspecific polymorphism data were available for both D. melanogaster (Zimbabwe, Africa) and D. ananassae (Bangkok, Thailand). For D. melanogaster, divergence was determined to D. simulans. For D. ananassae, D. atripex and/or D. phaeopleura were used for calculating divergence.

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Additional file 4:

Inference of ancestral sex-biased expression state of genes differing in expression between D. melanogaster (Dmel) and D. ananassae (Dana), using D. pseudoobscura (Dpse) as the outgroup. For 47 genes showing conflicting sex-biased classifications between D. melanogaster and D. ananassae, we inferred the ancestral expression state using microarray data from D. pseudoobscura.

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Additional file 5:

PCR and sequencing primers. Primers were designed to amplify genomic regions of 43 protein-coding genes. The PCR primers were also used as sequencing primers, with internal primers designed when necessary.

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Additional file 6:

All additional material. All files can be accessed through index.html, which can be viewed with any standard internet browser.

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