Table 2

Primers designed for this study.

Primer name

Primer sequence (5' - 3')

Region


Cytb-BopF1

TCA CAC AAA TTA TCA CAG GCC T

Cytochrome b

Cytb-BopF2

TCC TCC TAA CCC TAA TAG CAA C

Cytochrome b

Cytb-BopF3

CCT ACA CGA AAC AGG ATC AAA CAA

Cytochrome b

Cytb-BopF4

CTC CCC ATA TCA AAC CAG AAT GAT A

Cytochrome b

Cytb-BopR1

TCC GAC GAA GGC TGT TGC TAT TA

Cytochrome b

Cytb-BopR2

GGG GGT TGT TTG ATC CTG TTT C

Cytochrome b

Cytb-BopR3

TCG GAG GAT GGC GTA TGC AAA TAG

Cytochrome b

Cytb-BopR4

AAT GGA TGT TCG ACT GGT TGG CT

Cytochrome b

G3P-BopintF1

AAT CCC ACT GTG GAG TGA GAT TGT

GAPDH intron 11

G3P-BopintR1

AGG AGG CAG CTA CAG TAA TTT CAG GT

GAPDH intron 11

ODC-Bop-F2

CAG ACC CAG AGA CCT TTG TTC A

ODC intron 6 and 7

ODC-Bop-F3

GTA GCT TAC TTT GAC CAG CTT GGC A

ODC intron 6 and 7

ODC-Bop-R1

AGT TGC CAA TTT TAG TGC ATC AGT

ODC intron 6 and 7

ODC-Bop-R3

AAA CAG AGG TAA CTC ATG TTC AAG T

ODC intron 6 and 7


The primers have been designed to amplify short regions (~200-300 bp) from degraded DNA obtained from museum study skin samples.

Irestedt et al. BMC Evolutionary Biology 2009 9:235   doi:10.1186/1471-2148-9-235

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