Table 4 |
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|
Primers used for the quantification of fabp mRNA and hnRNA |
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|
Gene |
Forward primer |
Reverse primer |
||
|
|
||||
|
mRNA quantification |
AT1 |
Entrez gene ID |
||
|
fabp1a |
TAAGCTGACAGCGTTTGTGAAGGG |
AGATGCGTCTGCTGATCCTCTTGT |
60.0 |
791610 |
|
fabp1b.1 |
AAGCTGAAGGTGGTGCTGAACA |
CACGTTTGCTGATGCGCTTGTA |
58.0 |
554095 |
|
fabp1b.2 |
TGCCGTTCTCTGGGAAGTTTGAGT |
TGACTTTGTCTCCGCTCAGCATCT |
67.5 |
|
|
fabp7a |
TGTGCCACTTGGAAACTGGTTGAC |
AACATTGCCTACTTGCCTGGTAGG |
56.5 |
58128 |
|
fabp7b |
AAACCACTGCTGATGACCGACACT |
AGTGGTCTCTTTCCCATCCCACTT |
56.0 |
407736 |
|
fabp11a |
TGTGCAGAAACAGACCTGGGA |
ACAGCCACCACATCACCCATCTT |
56.0 |
447944 |
|
fabp11b |
GCTGTCACTACATTCAAGACCTG |
AGTTTACCATCCGCAAGGCTCA |
56.0 |
553579 |
|
ef1α |
TTGAGAAGAAAATCGGTGGTGCTG |
GGAACGGTGTGATTGAGGGAAATTC |
59.6 |
30516 |
|
hnRNA quantification |
||||
|
fabp1b.1 |
GAACTAACGTGTGCTGCTTGTG2 |
CACGTTTGCTGATGCGCTTGTA3 |
60.0 |
554095 |
|
fabp7a |
CCATCCATCAGATTTCTATGTGGG2 |
CATTATGCCTTCTCGTATGTGCG3 |
58.0 |
58128 |
|
fabp7b |
TTGGAAATGTGACCAAACCGACGC3 |
TCGTCTCGAAAGGGAATGCAGTGT2 |
59.0 |
407736 |
|
fabp11a |
CCAAGCCGTTTTTGATGATGTGAG2 |
GCTATTAATTTCCCATCCGACACC3 |
61.0 |
447944 |
|
ef1α |
AGCCTTGCATTCGTGCTGAAGT2 |
GGAACGGTGTGATTGAGGGAAATTC3 |
59.0 |
30516 |
|
|
||||
|
1 AT, annealing temperature Primer based on intronic2 and exonic3 sequences for gene-specific amplification. |
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|
Karanth et al. BMC Evolutionary Biology 2009 9:219 doi:10.1186/1471-2148-9-219 |
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