Table 4

Date of collection, number of isolated cells, method of DNA extraction and primer combination for each DNA sequence reported in this study.

Species

isolation date

# of cells

DNA extraction

primer combination

SSU

'Proterythropsis' sp. 1

24. June 2005

12

CTAB

PF1-R4

'Proterythropsis' sp. 2

25. June 2005

27

CTAB

PF1-R4

'Nematodinium' sp. 1

27. April 2006

2

Distilled water

PF1-R4

'Nematodinium' sp. 2

28. April 2006

3

Distilled water

PF1-R4

Warnowia sp. (BC)

02. May 2007

3

Kit

PF1-R4

"Warnowia sp." (Florida)

28. Aug. 2007

1

Sonication

EukA-EukB

LSU

'Nematodinium' sp.

28. April 2007

3

Kit

D1R-R2

Polykrikos kofoidii

28. April 2007

28

Kit

D1R-R2

Warnowia sp. (BC)

02. May 2007

3

Kit

D1R-R2

Polykrikos lebourae

18. May 2007

8

Kit

D1R-R2

'Pheopolyk.' hartmannii

31. July 2007

1

Sonication

Dino1662F-25R1

25F1-LSUR2

"1" and "2" after species names refer to different isolates; primers sequences are listed in Table 3.

Hoppenrath et al. BMC Evolutionary Biology 2009 9:116   doi:10.1186/1471-2148-9-116