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Open AccessResearch article

Muller's Ratchet and compensatory mutation in Caenorhabditis briggsae mitochondrial genome evolution

Dana K Howe email and Dee R Denver email

Department of Zoology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon, 97331, USA

author email corresponding author email

BMC Evolutionary Biology 2008, 8:62doi:10.1186/1471-2148-8-62

Published: 26 February 2008

Additional files

Additional File 1:

Alignment of ND5 and ψND5-1 elements from C. briggsae and Caenorhabditis sp. n. 5. This figure shows alignments of ND5 and ψND5-1 that were performed using ClustalW (gap parameters set to default: open = 10, extend = 5); residues conserved in all four sequences are in red. The sequence from isolate AF16 was used to represent C. briggsae and sequence from isolates JU727 represented Caenorhabditis sp. n. 5. The entire ψND5-1 sequences were input into the alignment program whereas input ND5 sequences included only the first 990 bp. As with C. briggsae, the ψND5-1 element in Caenorhabditis sp. n. 5 is located between the tRNAQ and tRNAF genes.

Format: TIFF Size: 377KB Download file

Additional File 2:

ND5 deletion heteroplasmy data. This supplementary table provides conventional PCR band scoring data and qPCR results for ND5 deletion heteroplasmy analyses.

Format: DOC Size: 168KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional File 3:

πNand πSvalues for mtDNA protein-coding genes. This supplementary table reports individual nucleotide diversity estimates used to calculate πN/πS ratios.

Format: DOC Size: 47KB Download file

This file can be viewed with: Microsoft Word Viewer


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