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Open AccessResearch article

Opossum carboxylesterases: sequences, phylogeny and evidence for CES gene duplication events predating the marsupial-eutherian common ancestor

Roger S Holmes1,2,3 email, Jeannie Chan1,2 email, Laura A Cox1,2 email, William J Murphy4 email and John L VandeBerg1,2 email

Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX, USA

Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, TX, USA

School of Biomolecular and Physical Sciences, Griffith University, Nathan, Queensland, Australia

Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA

author email corresponding author email

BMC Evolutionary Biology 2008, 8:54doi:10.1186/1471-2148-8-54

Published: 20 February 2008

Additional files

Additional file 1:

Controls for RT- PCR studies examining the expression of CES genes in opossum. The data presents RT-PCR controls showing that there were no RT-PCR products produced in the absence of reverse transcriptase (figure S1) compared with the results repeated here showing the RT-PCR products were formed when the experiment was conducted in the presence of reverse transcriptase (figure S2).

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