Evolution of GHF5 endoglucanase gene structure in plant-parasitic nematodes: no evidence for an early domain shuffling event

Additional file 1:

List of available data. Overview of all available endoglucanase gene sequences from nematode species and the most homologous endoglucanases from other eukaryotic and prokaryotic lineages that were included in our dataset. GenBank Accession Numbers and the type of available data are specified.

Format: XLS Size: 36KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 2:

Maximum Parsimony tree based on the intron data of GHF5 endoglucanases. Consensus tree of 34 Maximum parsimonious trees based on intron presence/absence data of GHF5 endoglucanases from different prokaryotic and eukaryotic lineages. Wagner Parsimony was applied. The bootstrap value is given on each node. The tree is rooted with the CBM of two genes from the fungus Trichoderma reesei.

Format: PDF Size: 6KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 3:

Intron positions of a typical PPN GHF5 endoglucanase in relation to the secondary gene structure. The position of alpha helices and beta strands was derived from comparison to known crystal structures of a GHF5 catalytic domain (1EGZC) and a CBM2 (1EXG). The signal peptide, catalytic domain, linker and CBM, are indicated in red, blue, yellow and green respectively. Transition zones are white. Arrows represent beta strands; boxes alpha helices. Introns are represented by a vertical black line and numbered in order of their occurrence, as specified in Figure 4.

Format: PDF Size: 195KB Download file

This file can be viewed with: Adobe Acrobat Reader