BMC Evolutionary Biology

official impact factor 3.70

Open Access Research article

Sex dependent imprinting effects on complex traits in mice

Reinmar Hager1*, James M Cheverud2, Larry J Leamy3 and Jason B Wolf1

Author Affiliations

1 Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK

2 Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, MO 63110, USA

3 Department of Biology, University of North Carolina at Charlotte, Charlotte, North Carolina 28223, USA

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BMC Evolutionary Biology 2008, 8:303 doi:10.1186/1471-2148-8-303

Published: 31 October 2008

Additional files

Additional file 1:

Sex-dependent imprinting effect QTL (SbiQTL) and their patterns for body weights, growth and organ weights. Coordinates (Mb) are based on mouse genome build 36 http://www.ensembl.org webcite and location refers to F2 cM map distances. Patterns are listed as Paternal = paternal expression, Maternal = maternal expression, Bipolar = bipolar imprinting, Under = polar underdominance, Over = polar overdominance, NS = non-significant. Loci were identified when the LPR for the overall effect of a locus exceeded the chromosome-wide or genome-wide threshold and they showed a significant sex by i interaction effect. The genome-wide significance threshold is given at an LPR of 3.41 and the chromosome-wide thresholds are given for each locus (in italics) as is the highest LPR for the locus. Traits shown in bold indicate a QTL effect.

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Additional file 2:

The table lists the identified sex by imprinting QTL (SbiQTL) and effects for males and females separately. First, the SbiQTL is identified, followed by the trait affected and by marker name, the F2 map location in cM and the genome coordinate based on mouse build 36 http://www.ensembl.org webcite along with the confidence intervals. This is followed by the locus LPR and the chromosome-wide threshold (the genome-wide is 3.41), the estimates for the sex by i interaction, the corresponding standard error, the interaction LPR and the r2 for the interaction effect. The next section details for males the additive a, dominance d, and imprinting i effects with their estimates, the estimates divided by the standard deviation and standard error as well as the corresponding LPR. Next, the imprinting patterns are given where 'Paternal' refers to paternal expression, 'Maternal' to maternal expression, 'Bipolar' to bipolar dominance, 'Over' to polar overdominance, 'Under' to polar underdominance and 'NS' to non-significant. This is followed by the genotypic values of the four ordered genotypes with their standard errors SE. We next present in the same order the equivalent information for females.

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