Structural and functional divergence of two fish aquaporin-1 water channels following teleost-specific gene duplication

doi:10.1186/1471-2148-8-259

BMC Evolutionary Biology

Volume 8

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Chauvigné F
Fabra M
Lozano J
Raldúa D
Cerdà J

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Research article

Structural and functional divergence of two fish aquaporin-1 water channels following teleost-specific gene duplication

Angèle Tingaud-Sequeira¹^,3 , François Chauvigné¹ , Mercedes Fabra¹^,4 , Juanjo Lozano² , Demetrio Raldúa¹^,5 and Joan Cerdà¹

¹Laboratory of Institut de Recerca i Tecnologia Agroalimentàries (IRTA)-Institut de Ciències del Mar, Consejo Superior de Investigaciones Científicas (CSIC), 08003 Barcelona, Spain

²Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 08036 Barcelona, Spain

³Génomique et Physiologie des Poissons, Université Bordeaux 1, UMR NuAGe, 33405 Talence, France

⁴Omnia Molecular, Barcelona Science Park, 08028 Barcelona, Spain

⁵Laboratory of Environmental Toxicology, Universidad Politécnica de Catalunya, 08220 Terrassa, Spain

author email corresponding author email

BMC Evolutionary Biology 2008, 8:259doi:10.1186/1471-2148-8-259


Published:	23 September 2008

Additional files

Additional file 1:

Alignment of the amino acid sequences of vertebrate AQP1 and teleost Aqp1a and Aqp1b. Alignment was performed using ClustalW employing the sequence from loop B to the start of the C-terminus, manually optimized using the Bioedit software. Conserved residues are shaded in black, residues conserved in at least 70% of the species in grey.

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Additional file 2:

Water permeability of X. laevis oocytes expressing sea bream wild-type (WT) or mutant Aqp1b. The Aqp1b-S238A, Aqp1b-S253A, Aqp1b-S258A and Aqp1b-S262A mutants are shown. (A) Water permeability of oocytes expressing 1 ng cRNA of WT Aqp1b or the different mutants. Permeability is expressed in % related to oocytes injected with wild-type Aqp1b. Values represent the mean ± SEM of 3 experiments (each performed with different batches of oocytes; n = 10–15 oocytes per treatment). (B) Immunoblots of total membrane equivalents of oocytes expressing WT or mutant Aqp1b showing that all proteins were expressed at similar levels. The apparent molecular mass of a 29-kDa marker is indicated on the left.

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Additional file 3:

Degenerate and gene- or cDNA-specific oligonucleotide primers used for cloning and RT-PCR analysis. The table list the oligonucleotide primers employed for the cloning of teleost AQP1-like cDNAs and sea bream aqp1a and aqp1b loci, and for RT-PCR analyses of aqp1b gene expression.

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Additional file 4:

Forward and reverse primers employed to introduce mutations into the sea bream Aqp1b cDNA. The table lists the oligonucelotide primers employed for the site-directed mutagenesis of the sea bream Aqp1b cDNA.

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