Phylogenetic analyses suggest multiple changes of substrate specificity within the Glycosyl hydrolase 20 family

Jari Intra , Giulio Pavesi and David S Horner

Dipartimento di Scienze Biomolecolari e Biotecnologie, Università di Milano, Via Celoria 26, 20133 Milano, Italy

author email corresponding author email

BMC Evolutionary Biology 2008, 8:214doi:10.1186/1471-2148-8-214


Published:	22 July 2008

Additional files

Additional file 1:

Eukaryotic GH20 protein sequences. All eukaryotic protein sequences of GH20 collected and used in this work.

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Additional file 2:

Prokaryotic GH20 protein sequences. All prokaryotic protein sequences of GH20 collected and used in this work.

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Additional file 3:

Divergent Eukaryotic GH20 protein sequences. Divergent eukaryotic protein sequences of GH20 collected and used in this work.

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Additional file 4:

Gene structure of metazoan β-hexosaminidases. Lengths of exons and introns of representative metazoan β-hexosaminidases.

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Additional file 5:

Alternative Hypotheses of relationships between major eukaryote GH20 clades. Schematic trees representing alternative hypotheses of relationships between major clades of eukaryotic GH20 family members. Hypotheses of relationships not excluded at the 5% confidence interval by the Shimodaira/Hasegawa test are depicted along with their log likelihoods, delta lnL and P-values. All analyses were performed with the WAG amino acid substitution model and 1 invariable and 4 gamma distributed site rate categories.

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