Unraveling the evolutionary history of the phosphoryl-transfer chain of the phosphoenolpyruvate:phosphotransferase system through phylogenetic analyses and genome context

Additional file 1:

Supplementary tables. Supplementary Table 1 lists the organisms included in this study. Supplementary Table 2 lists accession numbers and characteristics of the sequences utilized in this study.

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Additional file 2:

Supplementary figures. Figure S1: likelihood mapping analysis. Figures S2 and S3: maximum likelihood phylogenetic trees for EI and HPr sequences, respectively. Figure S4: schematic representation of the proposed ancestral rpoN gene cluster of Proteobacteria. Figure S5: comparison of the topologies of phylogenetic trees for 16S and EI sequences belonging to groups R, Ntr and T (VPES). Figure S6: phylogenetic reconstruction of 16S rRNA sequences of the strains used in this study harbouring genes encoding EI, HPr or HPrK. Figure S7: ptsH or ptsI gene clusters of Actinobacteria. Figure S8: gene clusters containing FPr encoding genes and related homologues. Figure S9: sequence alignment of the IIA^Fru and the intervening domain of FPr proteins, Acinetobacter sp. FruB protein, and the tandem IIA^Fru domains of Pseudomonas FruA proteins. Figure S10: ptsK gene clusters of Bacillales. Figure S11: ptsK gene clusters of Firmicutes and F. nucleatum. Figure S12: ptsH or ptsI gene clusters of Firmicutes, VPES, Borrelia and F. nucleatum.

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