Table 2

Ka/Ks values for each new Nosema/Vairimorpha RPB1 sequence, compared to that of V. necatrix.

Species pair

Non-synonymous positions

Non-synonymous differences

Ka

Synonymous positions

Synonymous differences

Ks

Ka/Ks


N. apis:V. necatrix

1437.58

229.25

0.1793

383.42

211.75

0.9999

0.1793

N. bombycis:V. necatrix

1434.67

259.58

0.2071

386.33

247.42

1.4426

0.1436

N. empoascae:V. necatrix

1427.75

250.17

0.1996

393.25

291.83

3.4155

0.0584

N. granulosis:V. necatrix

1427.58

262.58

0.2110

393.42

285.42

2.5655

0.0822

N. trichoplusiae:V. necatrix

1434.00

260.83

0.2083

387.00

249.17

1.4664

0.1420

V. cheracis:V. necatrix

1421.33

278.25

0.2269

399.67

372.75

-

-

V. cheracis:N. empoascae

1409.42

143.17

0.1091

411.58

283.83

1.8895

0.0577


Values of Ka/Ks considerably lower than 1 indicate relatively low levels of sequence polymorphism at non-synonymous sites, an indicator of strong purifying selection and hence of gene activity. The sequence of V. cheracis is too divergent from that of V. necatrix for Ka/Ks cannot be calculated, so an additional comparison is performed between V. cheracis and N. empoascae.

Ironside BMC Evolutionary Biology 2007 7:48   doi:10.1186/1471-2148-7-48

Open Data