|
PCR primers and annealing temperatures used to amplify 10 new markers |
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| Gene* |
Primers |
Sequences |
Annealing temp |
PCR steps |
|
|
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| zic1 |
zic1_F9 |
5' GGACGCAGGACCGCARTAYC 3' |
57 |
1st |
| zic1_R967 |
5' CTGTGTGTGTCCTTTTGTGRATYTT 3' |
PCR |
||
| zic1_F16 |
5' GGACCGCAGTATCCCACYMT 3' |
57 |
2nd |
|
| zic1_R963 |
5' GTGTGTCCTTTTGTGAATTTTYAGRT 3' |
PCR |
||
| myh6 |
myh6_F459 |
5' CATMTTYTCCATCTCAGATAATGC 3' |
53 |
1st |
| myh6_R1325 |
5' ATTCTCACCACCATCCAGTTGAA 3' |
PCR |
||
| myh6_F507 |
5' GGAGAATCARTCKGTGCTCATCA 3' |
62 |
2nd |
|
| myh6_R1322 |
5' CTCACCACCATCCAGTTGAACAT 3' |
PCR |
||
| RYR3 |
RYR3_F15 |
5' GGAACTATYGGTAAGCARATGG 3' |
55 |
1st |
| RYR3_R968 |
5' TGGAAGAAKCCAAAKATGATGC 3' |
PCR |
||
| RYR3_F22 |
5' TCGGTAAGCARATGGTGGACA 3' |
62 |
2nd |
|
| RYR3_R931 |
5' AGAATCCRGTGAAGAGCATCCA 3' |
PCR |
||
| Ptr |
Ptr_F458 |
5' AGAATGGATWACCAACACYTACG 3' |
55 |
1st |
| Ptr_R1248 |
5' TAAGGCACAGGATTGAGATGCT 3' |
PCR |
||
| Ptr_F463 |
5' GGATAACCAACACYTACGTCAA 3' |
62 |
2nd |
|
| Ptr_R1242 |
5' ACAGGATTGAGATGCTGTCCA 3' |
PCR |
||
| tbr1 |
tbr1_F1 |
5' TGTCTACACAGGCTGCGACAT 3' |
57 |
1st |
| tbr1_R820 |
5' GATGTCCTTRGWGCAGTTTTT 3' |
PCR |
||
| tbr1_F86 |
5' GCCATGMCTGGYTCTTTCCT 3' |
62 |
2nd |
|
| tbr1_R811 |
5' GGAGCAGTTTTTCTCRCATTC 3' |
PCR |
||
| ENC1 |
ENC1_F85 |
5' GACATGCTGGAGTTTCAGGA 3' |
53 |
1st |
| ENC1_R982 |
5' ACTTGTTRGCMACTGGGTCAAA 3' |
PCR |
||
| ENC1_F88 |
5' ATGCTGGAGTTTCAGGACAT 3' |
62 |
2nd |
|
| ENC1_R975 |
5' AGCMACTGGGTCAAACTGCTC 3' |
PCR |
||
| Gylt |
Glyt_F559 |
5' GGACTGTCMAAGATGACCACMT 3' |
55 |
1st |
| Glyt_R1562 |
5' CCCAAGAGGTTCTTGTTRAAGAT 3' |
PCR |
||
| Glyt_F577 |
5' ACATGGTACCAGTATGGCTTTGT 3' |
62 |
2nd |
|
| Glyt_R1464 |
5' GTAAGGCATATASGTGTTCTCTCC 3' |
PCR |
||
| SH3PX3 |
SH3PX3_F461 |
5' GTATGGTSGGCAGGAACYTGAA 3' |
55 |
1st |
| SH3PX3_R1303 |
5' CAAACAKCTCYCCGATGTTCTC 3' |
PCR |
||
| SH3PX3_F532 |
5' GACGTTCCCATGATGGCWAAAAT 3' |
62 |
2nd |
|
| SH3PX3_R1299 |
5' CATCTCYCCGATGTTCTCGTA 3' |
PCR |
||
| plagl2 |
plagl2_F9 |
5' CCACACACTCYCCACAGAA 3' |
55 |
1st |
| plagl2_R930 |
5' TTCTCAAGCAGGTATGAGGTAGA 3' |
PCR |
||
| plagl2_F51 |
5' AAAAGATGTTTCACCGMAAAGA 3' |
62 |
2nd |
|
| plagl2_R920 |
5' GGTATGAGGTAGATCCSAGCTG 3' |
PCR |
||
| sreb2 |
sreb2_F10 |
5' ATGGCGAACTAYAGCCATGC 3' |
55 |
1st |
| sreb2_R1094 |
5' CTGGATTTTCTGCAGTASAGGAG 3' |
PCR |
||
| sreb2_F27 |
5' TGCAGGGGACCACAMCAT 3' |
62 |
2nd |
|
| sreb2_R1082 |
5' CAGTASAGGAGCGTGGTGCT 3' |
PCR |
||
|
*Gene markers are named following annotations in ENSEMBLE. zic1, zic family member 1; myh6, myosin, heavy polypeptide 6; RYR3 (si:ch211-189g6.1), novel protein similar to vertebrate ryanodine receptor 3; Ptr (si:ch211-105n9.1), hypothetical protein LOC564097; tbr1, T-box brain 1; ENC1(559445 Entrezgene), similar to ectodermal-neural cortex 1; Glyt (zgc:112079), glycosyltransferase; SH3PX3, similar to SH3 and PX domain containing 3 gene; plagl2, pleiomorphic adenoma gene-like 2; sreb2, Super conserved receptor expressed in brain 2. | ||||
Li et al. BMC Evolutionary Biology 2007 7:44 doi:10.1186/1471-2148-7-44 |
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