Table 5

Primer sequences used to generate probes

Probe name

Primer name

Primer sequence (5' to 3')

Application

Rv1917c (and Rv1918c)

ppe-17

ttc aac tcc gtg acg tcg

Amplification of 471 bp 5' terminal region from Rv1917c and Rv1918c

ppe-18

cag cac acc ctt gga act g

Rv1753c

1753ISH_F

cgg tgg ctt tag tct acc tgc

Amplification of 279 bp 5' terminal region from Rv1753c

1753ISH_R

ccg gtc aat gtg tat ggg tg

mycP5 (Rv1796)

prot 5 f

gtg ctc gta atg tca tcg

Amplification of 658 bp of Rv1796

prot 5 r

cat atc ggc acc ata tcg

Gey van Pittius et al. BMC Evolutionary Biology 2006 6:95   doi:10.1186/1471-2148-6-95