Figure 10.

Southern hybridization analyses of the genomic DNA of 37 different species of the genus Mycobacterium. Summary of Southern blot results obtained with mycosin 5 (column 1) and PPE-MPTR probes (column 2 and 3) in comparison to previously-published results using MPTR and PGRS sequences, respectively (column 4 and 5), as indicated. Presence of hybridization signal is indicated by the word "Yes", while absence of signal is indicated by "No". The sign "-" indicates that hybridization was not performed in this species. Mycobacterial species are separated into fast- and slow-growing species (see Figure 3). * MPTR and PGRS hybridization results were obtained from previously-published studies by Hermans et al. [17], Ross et al. [75] and Poulet et al. [15]. ** data obtained from whole genome sequence information – see Table 1. *** negative results for Rv1917c and Rv1753c in M. marinum and M. ulcerans is in agreement with the genome sequencing data which indicated the absence of both of these genes within the genomes of this species. **** although previously published data indicated a failure of the MPTR repeat sequence to hybridize to the genomic DNA of these species, M. marinum- and M. ulcerans-specific PPE-MPTR genes have been identified in the current study through genome sequencing data.