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Evolution and expansion of the Mycobacterium tuberculosis PE and PPE multigene families and their association with the duplication of the ESAT-6 (esx) gene cluster regions

Nicolaas C Gey van Pittius1*, Samantha L Sampson2, Hyeyoung Lee3, Yeun Kim3, Paul D van Helden1 and Robin M Warren1

Author Affiliations

1 DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, US/MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa

2 Department of Molecular Microbiology and Infection, Centre for Molecular Microbiology and Infection, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK

3 Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Kangwon-do, Korea

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BMC Evolutionary Biology 2006, 6:95  doi:10.1186/1471-2148-6-95

Published: 15 November 2006

Additional files

Additional file 1:

M. ulcerans PE, PGRS and PPE genes. The data provided represent presence and absence of all orthologues of the members of the PE and PPE gene families of M. tuberculosis H37Rv in M. ulcerans (this file is the M. ulcerans equivalent to the data that is presented for M. avium paratuberculosis and M. leprae in Tables 3 and 4).

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Additional file 2:

Comparative genomics for gene size differences between M. tuberculosis H37Rv and CDC1551. The data in this table provide an overview of the reasons for size differences observed between annotated PE and PPE genes from the two M. tuberculosis genome databases, indicating that variation in size due to frameshifts, insertions and deletions is largely associated with the PE_PGRS and PPE-MPTR subfamilies.

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Additional file 3:

Comparative genomics for extent of sequence variation between M. tuberculosis H37Rv and CDC1551. The data in this table provide an overview of the extent of sequence variation on a protein level between the orthologues of the PE and PPE families in the two M. tuberculosis strains, indicating that the "ancestral-type" PE and PPE genes are highly conserved between strains, while the PPE-MPTR and PE_PGRS subfamilies are prone to sequence variation.

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