Southern blotting of SmTRC1. S. mansoni genomic DNA (5 μg) digested with the indicated restriction enzyme was loaded in each lane and analyzed by Southern blotting with a specific radiolabeled probe for SmTRC1. A parallel experiment was run with a probe for the Saci-2 retrotransposon , which was used as a benchmark. Probes of similar sizes and the same number of radioactive counts were used for each of the two hybridizations. Below the figure, the ratio between the total intensities of the SmTRC1/Saci-2 signals is indicated. This value was calculated for each digestion by integrating the signal from all the bands, and the average and total deviation was obtained by computing data from the two different digestions.
DeMarco et al. BMC Evolutionary Biology 2006 6:89 doi:10.1186/1471-2148-6-89