Table 5

Primer sequences used to amplify PRP8 intein encoding sequences from euascomycetes.

Primer name

Relevant species

Primer sequence

Aspfum-F1

A. fumigatus,

5' acagatgtcatccaagc 3'

Aspfum-F2

(including

5' tgggaaagagcatgccttgc 3'

Aspfum-F4

Afu. Var. ellipticus

5' gaaccaggaaatggagacg 3'

Aspfum-Rv1

and

5' ttcaacggtatcgtagcg 3'

Aspfum-Rv4

Neosartorya fischeri)

5' acagagtgaaccgacg 3'

Liu_1

Fumigati spp. and

5' atgaagagcaa [tc]cc [agct]tt [tc]tggtggac 3'

Liu_2

A giganteus

5' gcattcgtgag [tc]tt [ct]tt [ag]aa [tc]ttcat 3'

Ani-Fn

A. nidulans

5' agcttgtcttgccaac 3'

Ani-Fs

A. nidulans

5' acaaagacagaccaaccaataaacggattg 3'

Ani-Ra

A. nidulans

5' actgttatgcagtacaacatag 3'

Ani-Rsn

A. nidulans

5' tctcccaggagtctcgacgctaga 3'

Pb_Fwd1

P. brasiliensis

5' catatctctggaactgtggcag 3'

Pb_Fwd2

P. brasiliensis

5' gcagttgggtattgacacagtgc 3'

Pb_Rev1

P. brasiliensis

5' gcactgtgtcaatacccaactgc 3'

Pb_Rev2

P. brasiliensis

5' gcgttcgtcaacttcttgaacttcat 3'

Pb_Rev3

P. brasiliensis

5' gctttgtgctgcctcgttaacg 3'

Para_b-F3

P. brasiliensis

5' gatagaagtcgcacg 3'

Para_b-Rv4

P. brasiliensis

5' gggtcggtttatgc 3'

Butler et al. BMC Evolutionary Biology 2006 6:42   doi:10.1186/1471-2148-6-42