Table 2

Differences observed by comparison of grape chloroplast genome sequences with EST sequences obtained by BLAST search in Genbank.

Gene

Gene size (bp)

EST Sequencea

Number of variable sites

Variation typeb

Position(s)c

Amino acid change


atpI

744

1–744

2

G-A

C-U

453

629

A-A

L-S

clpP

591

62–366

5

G-A

A-U

64

65

D-I

T-A

A-U

70

71

Y-I

C-U

364

R-W

matK

1509

416–1262

2

C-U

G-A

448

1260

H-Y

K-K

ndhA

1092

1–553

1

T-C

553

L-L

ndhI

504

77–356

1

C-U

162

R-R

PetB

648

4–648

1

G-A

5

S-N

PetD

483

6–483

1

G-A

7

V-I

PsbA

1062

397–1014

2

T-C

G-A

420

463

R-R

A-T

rpl22

462

1–462

1

C-U

46

Q-Stop


aSequence analyzed coordinates based on the gene sequence, considering the first base of the initiation codon as bp 1. bVariation type: (nucleotide in genomic DNA) – (nucleotide in mRNA). cVariable position is given in reference to the first base of the initiation codon of the gene sequence.

Jansen et al. BMC Evolutionary Biology 2006 6:32   doi:10.1186/1471-2148-6-32

Open Data