Figure 2.

Potential single source-gene mutations in the most parsimonious ordering of the NANOG pseudogenes by source-gene mutation analysis. The left side depicts nucleotide sequences of the NANOG gene and pseudogenes after correction of post-H/C divergence mutations to the ancestral sequence. In two instances (sites 565 and 903), the ancestral sequence could not be determined, so both human and chimpanzee sequences are indicated with the human sequence on the left. At site 253, the human and chimpanzee sequences differ for NANOG, and the chimpanzee sequence is ancestral. However, we included the polymorphism because it explains the guanine in NANOGP8 (reverse arrow). Asterisks (*) denote post-insertion mutations and hyphens (-) denote deletions in the DNA sequences of the pseudogenes. The right side depicts inferred amino acid substitutions and the corresponding amino acids in the NANOG proteins of eight species: Cf = Canis familiaris, Ch = Capra hircus, Bt = Bos taurus, Rn = Rattus norvegicus, Mm = Mus musculus, Mf = Macaca fascicularis, Hs = Homo sapiens, Pt = Pan troglodytes. The "h" designation following a site number indicates that the site lies within the homeobox region.