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Morett E
Iturriaga G

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Iturriaga G
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Research article

Insights on the evolution of trehalose biosynthesis

Nelson Avonce¹^,2 , Alfredo Mendoza-Vargas¹ , Enrique Morett¹ and Gabriel Iturriaga²

¹Centro de Investigación en Biotecnología-UAEM, Av. Universidad 1001, Col. Chamilpa, Cuernavaca 62210, Mexico

²Instituto de Biotecnología-UNAM, Av. Universidad 2001, Col. Chamilpa, Cuernavaca 62210, Mexico

author email corresponding author email

BMC Evolutionary Biology 2006, 6:109doi:10.1186/1471-2148-6-109


Published:	19 December 2006

Additional files

Additional file 1:

List of the trehalose biosynthetic proteins detected in the complete sequenced organism. The taxonomic groups are shown on the left side; the accession numbers for each protein are indicated. Colours show each different protein type.

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Additional file 2:

Multiple alignment of TPS domains. The alignment was performed with CLUSTAL_X [32] and edited with the Seaview program [66]. The black arrows show the residues involved in the binding to glucose-6-phosphate. The black with white arrows show the residues that bind the UDP. The box shaded indicates highly conserved regions.

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Additional file 3:

Conservation of the OtsA active site along the evolution. All the proteins that conserved the complete active site of OtsA protein are labelled in blue.

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Additional file 4:

Multiple alignment of TPP domains. The alignment was performed with CLUSTAL_X [32] and edited with the Seaview program [66]. The black arrows show the active site residues in HAD superfamily. The shaded box indicates highly conserved regions.

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Additional file 5:

Conservation of the phosphatase consensus region along the evolution. All the proteins that conserved the complete active site are labelled in blue.

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Additional file 6:

Mutational substitution rate in TPS and TPP domains. The graphics show the difference between the non-synonymous (dN) and the synonymous (dS) substitution per codon (w = dN-dS). (A) TPS. (B) TPP.

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Additional file 7:

Genomic context of TPP and TPS domains. The TPS and TPP coding genes are neighbours in the genome, likely belonging to a single operon, are shown in orange. The TPS-TPP fusion proteins are labelled in black.

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Additional file 8:

Phylogenetic relationship between TPS and TPP domains. The TPS domains closely related to TPP domains according the genome context are label in yellow; TPS-TPP fusion proteins are labelled in black.

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