Figure 7.

Unrooted strict consensus tree of 3 equally parsimonious gene trees (1000 random sequence additions with TBR). Bootstrap proportions (500 bootstrap replicates, 10 random sequence additions each) for each hypothesized receptor group are indicated above or below the branch leading to the group. Bootstrap proportions for hypothesized receptor groups are provided only if greater than 50%. The 68% bootstrap proportion indicated by dashed lines corresponds to the entire group of vertebrate GnRHRs, a group not represented in the strict consensus tree. Class A GCPRs are shaded in blue, class B GPCRs are shaded in green, nematode orthologues are shaded in orange, vertebrate receptor sequence labels are black and invertebrate receptor sequence labels are white. Tree is derived from a 422 amino acid alignment of Class A and B GPCRs (See 4: GPCRs_alignment.pdf). GPCR abbreviations: akhr Adipokinetic hormone receptor; caccr Cardioacceleratory peptide receptor; calcr Calcitonin receptor; ccapr Crustacean cardioactive peptide receptor; corr Corazonin receptor; ghrhr Growth hormone releasing hormone receptor; gnrhr Gonadotropin releasing hormone receptor (types 1, 2, and 3); ortho1 AKHR/GnRHR orthologue 1; ortho2 AKHR/GnRHR orthologue 2; oxyr Oxytocin receptor; rhodlk Rhodopsin-like; rhod Rhodopsin; sctr Secretin receptor; vasr Vasopressin receptor (types 1A and 2). Organism abbreviations: Af Astyanax fasciatus (teleost fish); Ag Anopheles gambiae (mosquito); Am Apis mellifera (bee); Ami Alligator mississippiensis (alligator); Bm Bombyx mori (moth); Bt Bos Taurus (cattle); Ca Camponotus abdominalis (ant); Cb Caenorhabditis briggsae (nematode); Ce Caenorhabditis elegans (nematode); Cg Crassostrea gigas (oyster); Ci Ciona intestinalis (tunicate); Cj Callithrix jacchus (marmoset); Cp Cavia porcellus (guinea pig); Cph Caluromys philander (opossum); Cpi Culex pipiens (mosquito); Dm Drosophila melanogaster (fruit fly); Gg Gallus gallus (chicken); Hs Homo sapiens (human); Mm Mus musculus (mouse); Mo Microtus ochrogaster (vole); Ms Manduca sexta (moth); Oc Oryctolagus cuniculus (rabbit); Ol Oryzias latipes (teleost fish); Pa Periplaneta americana (cockroach); Pc Procambarus clarkii (crustacean); Rc Rana catesbeiana (bull frog); Rn Rattus norvegicus (rat); Ss Sus scrofa (pig); Tc Tribolium castaneum (beetle); Tn Typhlonectes natans ("rubber eel", amphibian); Tni Tetraodon nigroviridis (teleost fish); Tv Trichosurus vulpecula (opossum).