Figure 6.

Nuclear localization of Ce-GnRHR to the germline and intestinal cells. Permeabilized adult N2 worm probed with anti-Ce-GnRHR antibody (i) and Hoechst stain (ii). Nuclear localization of Ce-GnRHR to oocytes was clearly demonstrated following superimposition of images i and ii (iii). Permeabilized adult N2 worm probed with anti-Ce-GnRHR antibody (iv) and Hoescht stain (v). Nuclear localization of Ce-GnRHR to the intestinal cells was clearly demonstrated following superimposition of images iv and v (vi). A high magnification image of a superimposed nucleus is presented in the insert of vi. A superimposed image of the gonadal arm of an adult N2 worm is shown in vii. Higher magnification images of the gonadal arm stained with anti-Ce-GnRHR antibody (viii), Hoechst stain (ix) and superimposed (x) demonstrates Ce-GnRHR staining is primarily confined to the nuclear membrane. Scale: 50μm.