Identification of a gonadotropin-releasing hormone receptor orthologue in Caenorhabditis elegans
- Equal contributors
1 Section of Geriatrics and Gerontology, Department of Medicine, University of Wisconsin and Geriatric Research, Education and Clinical Center, Veterans Administration Hospital, Madison, WI 53705, USA
2 Biotechnology Center, University of Wisconsin, Madison, WI 53705, USA
3 Case Western Reserve University, Cleveland, OH 44106, USA
BMC Evolutionary Biology 2006, 6:103 doi:10.1186/1471-2148-6-103Published: 29 November 2006
Additional file 1:
Prediction of transmembrane domains (TMs) in human GnRHR1 and Ce-GnRHR. The sequence alignment of human GnRHR1 and Ce-GnRHR presented here is taken from Figure 1; Thus for consistency, some sites are represented by gaps in both sequences. Functionally important residues (FIRs) are colored (see legend for classification). Overall, there is 56 % similarity between the FIRs of human GnRHR1 and Ce-GnRHR. TMs for both human GnRHR1 and the Ce-GnRHR were predicted using multiple programs described in Methods and are represented as colored lines above/below the alignment. Each program predicted Ce-GnRHR, like human GnRHR1, to have 7 TMs.
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Additional file 2:
Expression of the GnRHR1 orthologue in C. elegans. Worm (N2) homogenates were immunoblotted with a monoclonal antibody against human GnRHR1. The antibody recognized a 46-kDa band, the calculated molecular weight of the GnRHR1 orthologue. The secondary antibody control did not show immunoreactivity. The molecular weight markers are indicated in kDa on the left.
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Additional file 3:
Comparison of sequence and genomic organization between Ce-GnRHR and the putative orthologue to human GnRHR2 (orthologue 2). (A). Pairwise sequence alignment of Ce-GnRHR and orthologue 2 [GenBank; NP_506566]. The Ce-GnRHR sequence used to raise the anti-Ce-GnRHR antibody is highlighted with a green box. (B). Comparative genomic organization of Ce-GnRHR and orthologue 2. Exon colors in orthologue 2 sequences correspond to homologous regions in the Ce-GnRHR sequence. The gray box delimits the C-terminus portion of Ce-GnRHR absent from orthologue 2.
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