Interaction of Arabidopsis IQD20 and calmodulin in vitro. Calmodulin-agarose beads were incubated in the presence of Ca2+ or absence of Ca2+ (+EGTA) with soluble proteins prepared from induced bacterial cultures expressing a T7-tagged IQD20 protein and treated as described in Methods. Proteins of the total bacterial extract, the supernatant fraction, the entire pellet (beads) fraction, and of the last wash were resolved by SDS-PAGE, transferred to a membrane, and probed with a HRP conjugated T7-Tag monoclonal antibody.
Abel et al. BMC Evolutionary Biology 2005 5:72 doi:10.1186/1471-2148-5-72