Summary of the major transpositions occurring in the mitochondria of whiteflies and the relationship of these changes to the phylogeny of whitefly species. The phylogenetic tree was obtained on the basis of combined mitochondrial cytB-N22-16S rDNA and Portiera endosymbiont 16S* and 23S* rDNA using the maximum likelihood method. Numbers at nodes correspond to bootstrap values after 500 replicates. The combination of the host and endosymbiont sequence data is justified by their cospeciation . A, B, C, D indicate transposition type; Y, indicates mitochondria with an ancestral gene arrangement. Large arrowhead in mitochondrial genome indicates the original position of the transposed genes. Small arrowheads indicate the position of the insertion of the genes. Arrows outside circle indicate the direction of the transcription of the transposed genes. Arrow by the arrowhead of the B type transposition indicates the changed direction of transcription of the 12S rDNA. tRNAs have been omitted. (*), by species names indicate that the full mitochondrial genome was sequenced. (+), by species names indicates that a DNA fragment containing all or a part of the gene encoding for COIII and adjacent genes was sequenced. (o), by species name indicates that using oligonucleotide primers to COII and ND5 a PCR product was obtained corresponding to a size that was consistent with the presence of COIII-(tRNA-G)-ND3-(tRNAs-A-R-N) in the ancestral position (Fig. 8).
Thao et al. BMC Evolutionary Biology 2004 4:25 doi:10.1186/1471-2148-4-25