Email updates

Keep up to date with the latest news and content from BMC Evolutionary Biology and BioMed Central.

Open Access Research article

An evolutionary ratchet leading to loss of elongation factors in eukaryotes

Gemma C Atkinson123*, Anton Kuzmenko14, Ivan Chicherin4, Axel Soosaar1, Tanel Tenson1, Martin Carr5, Piotr Kamenski4 and Vasili Hauryliuk123

Author Affiliations

1 University of Tartu, Institute of Technology, Nooruse 1, 50411 Tartu, Estonia

2 Department of Molecular Biology, Umeå University, Umeå, Sweden

3 Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden

4 Department of Molecular Biology, Faculty of Biology, Moscow State University, Moscow, Russia

5 School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UK

For all author emails, please log on.

BMC Evolutionary Biology 2014, 14:35  doi:10.1186/1471-2148-14-35

Published: 24 February 2014

Abstract

Background

The GTPase eEF1A is the eukaryotic factor responsible for the essential, universal function of aminoacyl-tRNA delivery to the ribosome. Surprisingly, eEF1A is not universally present in eukaryotes, being replaced by the paralog EFL independently in multiple lineages. The driving force behind this unusually frequent replacement is poorly understood.

Results

Through sequence searching of genomic and EST databases, we find a striking association of eEF1A replacement by EFL and loss of eEF1A’s guanine exchange factor, eEF1Bα, suggesting that EFL is able to spontaneously recharge with GTP. Sequence conservation and homology modeling analyses indicate several sequence regions that may be responsible for EFL’s lack of requirement for eEF1Bα.

Conclusions

We propose that the unusual pattern of eEF1A, eEF1Bα and EFL presence and absence can be explained by a ratchet-like process: if either eEF1A or eEF1Bα diverges beyond functionality in the presence of EFL, the system is unable to return to the ancestral, eEF1A:eEFBα-driven state.

Keywords:
eEF1A; EFL; eEF1B; Ribosome; Elongation factor; GTPase; GEF; Molecular evolution; Eukaryotes