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Open Access Research article

Multiple hybridization events, polyploidy and low postmating isolation entangle the evolution of neotropical species of Epidendrum (Orchidaceae)

Isabel Marques123*, David Draper2, Lorena Riofrío2 and Carlos Naranjo2

Author Affiliations

1 Department of Agriculture (Botany), High Polytechnic School of Huesca, University of Zaragoza, C/ Carretera de Cuarte Km 1, Huesca E22071, Spain

2 Departamento de Ciencias Naturales, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja 1101608, Ecuador

3 Current address: UBC Botanical Garden & Centre for Plant Research, and Department of Botany, University of British Columbia, 3529-6270 University Blvd, Vancouver BC V6T 1Z4, Canada

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BMC Evolutionary Biology 2014, 14:20  doi:10.1186/1471-2148-14-20

Published: 4 February 2014

Abstract

Background

Hybridization and polyploidy are central processes in evolution and speciation. These mechanisms often lead to complex patterns of genetic variation and the creation of novel genotypes, which may establish if they become isolated from gene flow. However, in the absence of reproductive isolation, species boundaries might easily be disrupted. Here, we used a combination of AFLPs, chloroplast DNA markers and flow cytometry to investigate the evolutionary outcomes of hybridization between two endemic Ecuadorian species of Epidendrum (E. madsenii and E. rhopalostele) in three hybrid zones. Postmating isolation was also quantified to determine the role of this barrier in restraining gene flow between hybrids and the parental species. In addition, future ecological niche models were constructed to predict the outcomes of hybridization between these species.

Results

Our results confirmed the presence of hybrids in all hybrid zones, but revealed that a third parental species (E. falcisepalum) has contributed to one of the hybrid zones studied. Backcross genotypes were frequent in all hybrid zones, which was in accordance with the absence of strong reproductive barriers. The process of hybridization was highly asymmetric and followed in some cases by polyploidy. The projection of future niche models predicted a severe reduction in the area suitable for the occurrence of these species, although favorable conditions will still occur for the existence of the current hybrid zones.

Conclusions

The recurrent process of hybridization has compromised the genetic integrity of the parental species. Most individuals of the parental species can no longer be considered as pure-bred individuals because most were classified as backcrossed hybrids. Novel genetic lineages occur in all hybrid zones implying that hybrids are fertile and can compete with the parental species. These results, together with the prediction of suitable conditions for the future occurrence of these hybrid zones, highlight the importance of conserving these geographic areas as sources of novel taxonomic entities.

Keywords:
AFLPs; Conservation; Genome size; Orchids; Reproduction; Reticulate evolution