Table 6

Human kinases which are predicted to phosphorylate specific residues in human sirtuins
Kinase Function Localization Sirt-1 Sirt-2 Sirt-3 Sirt-4 Sirt-5 Sirt-6 Sirt-7
BTK B lymphocyte development, differentiation and signaling. Cytoplasm, Cell membrane, Nucleus. 121-Y
    104-Y
CDK1 Control of the eukaryotic cell cycle. Nucleus, Cytoplasm, Mitochondrion.
    14-S 27-S 47-S 530-T 540-S
682-S
218-T 280-T
    368-S 372-S
150-T 36-S 87-T 178-S
    294-T 303-S 330-S 338-S
345-T
CDK2 Control of the cell cycle Cytoplasm, Cytoskeleton, Centrosome, Nucleus, Cajal body, Cytoplasm, Endosome.
    14-S 27-S 47-S 530-T 540-S
545-S 682-S
280-T 365-T
    368-S 372-S
294-T 303-S 330-S 338-S
CDK4 Regulate the cell-cycle during G1/S transition. Cytoplasm, Nucleus, Nuclear Membrane.
    14-S 27-S 530-T 540-S
545-S
98-S 279-S
    368-S 372-S
294-T 303-S 330-S 338-S 345-T 377-S
CDK5 Neuronal cell cycle arrest and differentiation Nucleus, Cell membrane, Cytoplasm.
    27-S 530-T 540-S
545-S
218-T
    368-S
    294-T 303-S 330-S 338-S
CDK6 Control of the cell cycle and differentiation Cytoplasm, Nucleus.
    14-S
545-S
280-T
    372-S
329-S
CDK7 Cell cycle control and in RNA polymerase II-mediated RNA transcription. Nucleus, Cytoplasm, Perinuclear region.
    27-S
344-T 545-S 549-S 605-S
    747-S
166-T 94-T
    294-T
355-S
148-T 400-T
DYRK1 Regulation of DNA damage response and regulation of protein deacetylation Interchromatin regions of nucleoplasm
    14-S
111-S
    538-S
744-S
    747-S
53-S 249-S
    372-S
117-S
    330-S 338-S
355-S
EGFR Activate signaling cascades; involved in cell-cell adhesion, regulation of apoptotic process, regulation of MAPK Cell membrane, Endoplasm, Golgi apparatus membrane Nucleus membrane, Endosome membrane. 642-Y 699-Y
    74-Y
JAK1 Involved in the IFN-alpha/beta/gamma signal pathway. Endomembrane system, Peripheral membrane protein. 699-Y 742-Y
LCK Developing T-cells and function of mature T-cells in thymus Cytoplasm, Cell membrane, Lipid rafts. 121-Y 658-Y 699-Y 742-Y
    104-Y
165-Y
MAPK3 MAPK signal transduction pathway Cytoplasm, Nucleus.
    27-S 47-S 530-T 540-S
218-T 280-T
    368-S 372-S
36-S 176-T
    294-T 303-S 330-S 338-S
345-T
MAPK8 Cell proliferation, differentiation, migration, transformation and programmed death. Cytoplasm, Nucleus.
    27-S 530-T 540-S
545-S
    368-S
36-S
    294-T 330-S 338-S
MAPK9 Cell proliferation, differentiation, migration, transformation and programmed death. Cytoplasm, Nucleus.
    27-S 530-T 540-S
682-S
280-T
    368-S 372-S
    330-S 338-S
MAPK10 Neuronal proliferation, differentiation, migration and programmed death. Cytoplasm, Membrane, Lipid-anchor, Nucleus.
    14-S 27-S 540-S
682-S
    368-S 372-S
    303-S 330-S 338-S
MAPK11 MAPK pathway and role in cellular responses to proinflammatory cytokines or physical stress Cytoplasm, Nucleus.
    540-S
545-S 682-S
98-S 280-T
MAPK12 Role in cellular responses to proinflammatory cytokines or physical stress Cytoplasm, Nucleus, Mitochondrion.
    14-S 27-S 540-S
545-S
280-T
    368-S
150-T
PAK1 T cell receptor signaling pathway, apoptotic process, regulation of cell proliferation Cell-extracellular matrix contact, Cytoplasm.
    615-S
PAK2 Cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Nuclues, Cytoplasm, Cell membrane.
    615-S

PKD1

(PRKD1)

DAG signaling, regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) Cytoplasm, Cell membrane, Golgi apparatus. 152-T 167-T
PLK1 Cell cycle, centrosome maturation and spindle assembly, removal of cohesins from chromosome arms, anaphase-promoting complex, regulation of mitotic exit and cytokinesis. Nucleus, Kinetochore, Centrosome, Cytoskeleton.
    538-S 540-S
550-S
    23-S 25-S
365-T
    368-S
383-T
    294-T 330-S
400-T
PRKCA Organizes subcellular localization of membrane proteins containing PDZ sequence, clustering of receptors, synaptic plasticity. Perinuclear region, Synapse. 383-T 337-S 229-T
ZAP70 Regulation of adaptive immune response, motility, adhesion and cytokine expression of mature T-cells, thymocyte development. development and activation of primary B-lymphocytes. Cytoplasm, Cell membrane. 121-Y 699-Y

[In red the kinases acting on N-terminal residues, in green on cores and in light blue on C-terminal. Phosphosites predicted but already experimentally determined are indicated underlined and in bold. In the table known functions and cellular locations exerted by these kinases are also reported [Shchemelinin et al., 2006].

Costantini et al.

Costantini et al. BMC Evolutionary Biology 2013 13:60   doi:10.1186/1471-2148-13-60

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