Additional file 1: Table S1.
List of primers designed for detection of α-amylase genes orthologous to Phchr1|7087|. PCR conditions were: initial denaturation 94°C, 6 mn; denaturation 94°C, 25 s; annealing 58°C, 50s; elongation 72°C, 1 mn, 45 cycles using the Taq Gold polymerase (Applied Biosystems). Various combinations of forward and reverse primers were tried.
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Da Lage et al. BMC Evolutionary Biology 2013 13:40 doi:10.1186/1471-2148-13-40